Gradual transitions in genetics and songs between coastal and inland populations of Setophaga townsendi

Setophaga townsendi is a species of wood-warbler (family Parulidae) in northwestern North America that has a geographic structure in the mitochondrial and nuclear genomes: while interior populations have differentiated mitonuclear ancestry from the sister species S. occidentalis, coastal populations have a mix of inland and S. occidentalis mitonuclear ancestries. This coastal-to-inland transition in genomic ancestry raises the possibility of similar geographic structure in phenotypic traits, especially those involved in mate choice. Using qualitative and multivariate approaches, we investigated whether there is a sharp transition between coastal and inland populations in both songs and nuclear DNA. We find there is a shallow geographic cline in the Type I song but not in the Type II song. Nuclear DNA shows a gradient between the coast and inland. There is little correlation between variation in song and the isolation-by-distance pattern in the nuclear DNA. The learned songbird song is s..., Data collection
Song recordings were collected at 30 locations across British Columbia from May to July of 2017, using a Marantz PMD660 digital recorder and an Audio-Technica 815a Shotgun microphone. Recordings were typically eight to ten minutes long and consisted of ten to forty songs. For songs recorded after June 25th, a playback of song recordings was used to encourage birds to sing. We designed playbacks to consist of three song variants from different regions of the S. townsendi range, to avoid playback matching. 
This dataset consists of songs of 249 birds (180 from field recordings, 39 from Xeno-Canto, and 30 from Macaulay Library). For each bird, songs were characterized into types based on visual similarity and the results of Janes and Ryker (2016) and Janes (2017). We classified the clear song as the Type I song (i.e., used more in female attraction), and the buzzy song as the Type II song (i.e., used more in territorial defense).
We randomly selected three numbers from the ..., , # Gradual transitions in genetics and songs between coastal and inland populations of *Setophaga townsendi*

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This dataset consists of song recordings that were collected at 30 locations across British Columbia from May to July of 2017, using a Marantz PMD660 digital recorder and an Audio-Technica 815a Shotgun microphone.

Three songs of each song type were analyzed using Raven Pro 1.4 for an analysis of song variation across the range of *Setophaga townsendi*.

These song variables were used in a PCA to quantify song variation.

## Description of the Data and file structure

This dataset consists of songs of 249 birds (180 from field recordings, 39 from Xeno-Canto, and 30 from Macaulay Library). For each bird, songs were characterized into types based on visual similarity and the results of Janes and Ryker (2016) and Janes (2017). We classified the clear song as the Type I song (i.e., used more in female attraction), and the buzzy song as the Type II song (i.e., used more in territ...