KPC pancreatic cancer cells are a novel immunocompetent murine model supporting human adenovirus replication and tumor oncolysis. Transcriptomic Analysis of Adenoviral Replication in Murine KPC-I and CMT64.6 Cancer Cells.

The possibility to use immunocompetent mouse models to study Oncolytic adenovirus antitumor efficacy has been hindered by the limited ability of human adenovirus to replicate in murine cells. In this work we identified KPC-I murine cells susceptible to adenoviral replication. The study also aimed to understand the mechanism that supported adenoviral replication in murine KPC-I cells and not in other murine cells such as CMT64.6 cells. KPC-I and CMT64.6 murine cancer cells were infected or not with 100 MOI of the adenovirus Adwt-E and bulk RNAseq analysis was conducted. Results showed a subset of deregulated genes in response to adenoviral infection in KPC-I but not in CMT64.6 cells, highlighting the pivotal role of calcium signaling and genes involved in autophagy. Our data suggest that KPC-I cells permissiveness to oncolytic adenovirus could be related to higher susceptibility of KPC-I cells to autophagy-mediated cell lysis.