Response of the mature nodules of the Medicago truncatula/sinorhizobium medicae md4 symbiotic association to systemic signaling of plant N deficit. Response of the mature nodules of the Medicago truncatula/sinorhizobium medicae md4 symbiotic association to systemic signaling of plant N deficit

The experiment aims to identify the response of mature nodules of Medicago truncatula /Sinorhizobium medicae md4 to the systemic signaling of plant N deficit. The root systems of nodulated symbiotic plants were divided into two compartments. The N source of N2 fixing plants was exclusively the air. To reduce the plant nitrogen (N) supply (N-deficient conditions), we flushed one half root system with Ar/O2 (80/20 v/v) while the other half root system was kept on control conditions (Air). To characterize the systemic responses of the transcriptome to plant N deficit , nodules and roots of the non-treated half root system were harvested and total RNA isolated for RNAseq analysis. We performed a time-course (6 hours, 1-, 3- and 5-days treatment).