Expression data from 15-day-old mouse testes

Activation of the Heat Shock Factor 1 (HSF1) in spermatogenic cells leads to apoptosis and infertility of males. To elucidate a mechanisms of apoptosis induced by HSF1 we generated transgenic mice expressing mutated, constitutively active human HSF1 (with a deletion of amino acids 221–315) specifically in spermatocytes (under control of the rat Hspa2 promoter). We carried out genome-wide transcriptional analyses in testes of control, wild-type, and transgenic males. Genes that changed the expression due to activation of HSF1 have been identified. On Affymetrix gene chip arrays we analyzed RNA isolated from testes of 15-day-old FVB/N mice (corresponding to the first wave of the meiotic division and beginning of the transgene expression), wild-type and transgenic with active HSF1 transcription factor.