TABLE 2 in Comparing whole-genome shotgun sequencing and DNA metabarcoding approaches for species identification and quantification of pollen species mixtures

TABLE 2 Composition of artificial pollen mixtures used in this study Mixture typeMixture numberSpeciesRelative proportions in mixture (based on number of pollen grains)Relative proportions in mixture (based on estimates of DNA quantity)Sequencing facility and platformIncreasing species richness (2–9)1Broussonetia papyrifera, Artemisia tridentata, Zea mays0.323 5, 0.450 4, 0.2260.084 3, 0.685 9, 0.229 8EIGC HiSeq2Broussonetia papyrifera, Bassia scoparia, Artemisia tridentata, Poa pratensis, Zea mays0.445 2, 0.089 7, 0.231 5, 0.173 7, 0.060.138 0, 0.044 5, 0.419 1, 0.326 0, 0.072 4EIGC HiSeq3Populus tremuloides, Broussonetia papyrifera, Carya illinoinensis, Bassia scoparia, Artemisia tridentata, Poa pratensis, Zea mays0.136 5, 0.244 8, 0.137 3, 0.071 5, 0.194 1, 0.137 5, 0.0780.033 7, 0.084 7, 0.056 3, 0.039 6, 0.392 2, 0.288 0, 0.105 6GGC MiSeq4Populus tremuloides, Populus deltoides, Broussonetia papyrifera, Carya illinoinensis, Bassia scoparia, Ambrosia artemisiifolia, Artemisia tridentata, Poa pratensis, Zea mays0.178 2, 0.178 2, 0.157 1, 0.069 5, 0.028, 0.13, 0.232 7, 0.080 2, 0.0460.045 8, 0.045 8, 0.056 5, 0.029 6, 0.016 1, 0.077 5, 0.489 2, 0.174 8, 0.064 7GGC MiSeqDecreasing taxonomic relatedness (congeneric-different orders)5Populus deltoides, Populus tremuloidesUnknownGGC MiSeq6Poa pratensis, Zea mays0.491 9, 0.5080.600 6, 0.399 4GGC MiSeq7Broussonetia papyrifera, Artemisia tridentata0.411, 0.5890.106 7, 0.893 3GGC MiSeq8Broussonetia papyrifera, Poa pratensis0.538 8, 0.461 20.161 7, 0.838 3GGC MiSeqIncreasing rarity of Broussonetia papyrifera (0.5388–0.089 7 of pollen grains; 0.1617–0.016 0 of DNA in pollen grains)8Broussonetia papyrifera, Poa pratensis0.538 8, 0.461 20.161 7, 0.838 3GGC MiSeq9Broussonetia papyrifera, Poa pratensis0.151 4, 0.848 60.028 6, 0.971 4GGC MiSeq10Broussonetia papyrifera, Poa pratensis0.089 7, 0.910 30.016 0, 0.984 0GGC MiSeq11Broussonetia papyrifera, Poa pratensis0.094 5, 0.905 50.016 9, 0.983 1GGC MiSeq