RNAseq Analysis Investigates Transcriptional Similarities and Differences Between Cryopreserved Parental and Engineered iPSC Derived Macrophages

RNA-seq analysis was used to investigate the transcriptional profile to reflect the changes in the parental vs engineered iPSC-derived macrophages. The RNA seq analysis provided an insight to uncover underlying mechanisms that might be triggered by perturbations in SNCA, Progranulin, and MeCP2 genes in iPSC derived macrophages. RNA seq data revealed an upregulation of 431 genes and concomitant downregulation of 276 in SNCA A53T Macrophages, an upregulation of 549 genes and concomitant downregulation of 356 in GRN R493X macrophages, an upregulation of 466 genes and concomitant downregulation of 406 genes in MeCP2HM macrophages when compared to the parental unengineered macrophages. These genes affected pathways regulating cell metabolism, exocytosis, transport, degranulation, immune activation, vascular circulation and neural differentiation and provided a comprehensive analysis of all cellular pathways perturbed by genetic engineering in SNCA, Progranulin, and MeCP2 genes. Investigation of perturbations resulting from genetic engineering mimicking neurological diseases; Parkinson's disease (SNCA A53T), Neuronal Ceroid Lipofuscinosis (GRN R493X), and Rett Syndrome (MECP2). >>> Raw data have not been provided for this study. Submitter indicates the risk of re-identifying the donor is against FCDI privacy policy. <<<<