Bulk transcriptomic profiling of eosinophils from skin and bone marrow during atopic dermatitis. Bulk transcriptomic profiling of eosinophils from skin and bone marrow during atopic dermatitis

Atopic dermatitis was induced on dorsal skin of 7-10 week-old C57BL/6 female mice by bi- or tri-daily MC903 treatment. On day 8, viable mouse eosinophils (CD45+F4/80+SiglecF+) were sorted from skin regions of healthy or vehicle/treated atopic dermatitis or femur/tibia-derived bone marrow by FACS. Samples were paired within individual mice (4 mice each). RNA from 500 cells were isolated and profile for transcriptome by sequencing. Overall design: Dermal and bone marrow eosinophils were sorted by surface antigens by flow cytometry followed by transcriptomic profiling with bulk RNA-seq.