Expression of genes involved in glycosylation pathway in mouse denrtic cells.. Expression of genes involved in glycosylation pathway in mouse denrtic cells.

These datasets uncover the regulation of glycosylation genes by TGF-beta in DC2.4 cells and dendritic cells (DCs) isolated from mice harboring MCA101 fibrosarcoma tumors. Overall design: Single cells suspensions from spleen and tumor (MCA101) were stained with antibodies directed against CD11c, MHC-II, CD103, CD8, F4/80, CD11b and CD45, and CD103-pos/CD8-pos DC1 cells purified using FACS sorting. DC2.4 cells were collected upon trypsinization after 4 days of 2.5 ng/ml TGF-beta stimulation. Expression of genes involved in glycosylation were quantified by quantitative real-time PCR (qRT-PCR) using the glycosylation RT2 Profiler PCR Array according to the manufacturer’s instructions (PAMM-046Z, Qiagen). Briefly, RNA was isolated using the RNeasy Kit (Qiagen), and 50-100 ng RNA was converted to cDNA using the RT2 First Strand Kit (Qiagen). The cDNAs were subjected to amplification using gene-specific primers and SYBR Green (RT2 SYBR Green Master Mix, Qiagen) in an Ab7100 Real-Time PCR System (Thermo Fisher Scientific).