Synchronized cell analysis of ΔseqA, ΔmukB and parE10 strains.

(A) Timing of replication and segregation of the gln locus. Cells were synchronized by baby machine in minimal alanine media at the indicated temperature and assayed for gln copy number (grey triangles) and number of TetR-YFP foci per cell (black circles) during the cell cycle. Values are means of two experiments ±1 SD. Dashed lines indicate the cumulative percentage of cells in the synchronized fraction that have replicated and segregated the gln locus (right ordinate), with times at 50% shown in minutes after birth. (B) Cell cycle diagrams are shown based on the timing of oriC, gln and ter duplication by qPCR (Materials and Methods). Doubling times in minimal alanine media were: WT/30°C, 119 min; WT/37°C, 98 min; ΔseqA/30°C, 134 min; ΔmukB/30°C, 211 min; parE10/30°C, 147 min; parE10/37°C, 174 min.