Genetic interaction of fus3Δ with septin associated genes.

All of strains were BY474-derivative haploids and grown to exponential phase in liquid YPD at 30°C unless stated otherwise. (A) Schematic representation of Elm1p substrates and a morphogenesis checkpoint. For details, see Introduction. (B-D) Indicated strains (MY8092, 13826, 13827, 13829, 13830, 13832, 13833, 12156, 12871, 12886, 12941, 12944, 12990, 13125) was photographed. (E) Indicated cells (MY8092, 12886, 12960 and 12961) were serially diluted on YPD and incubated at either 23°C for 2 days or 37°C for 1 day. (F) MY12960 and 12961 cells grown to exponential phase at 23°C were transferred to 37°C for 12 hr. Cells were fixed with 3.7% formaldehyde and stained with DAPI. (B-D, and F) Bar, 10 μm.