Effect of knockdown or overexpression of CYP1B1 on gene expression during proliferation process of colorectal cancer cells (RKO and SW480).

To investigate the function of CYP1B1 in the regulation of proliferation process, we established SW480 cells in which target gene has been knocked down by shRNA and established RKO cells in which target gene has been upregulated by overexpressed plasmid. Overall design: We performed gene expression profiling analysis using data obtained from RNA-seq of 4 different cells (RKO-Control vs RKO-CYP1B1, SW480-shNT vs SW480-shCYP1B1).