Original data

Our study elucidates a partial mechanism underlying inflammation in busulfan‑induced oligozoospermic mice, implicating the S100A9/TLR4/NF‑κB pathway in testicular inflammation. Administration of Tyr significantly reduces S100A9 and TLR4 expression in the testis, thereby ameliorating inflammation. Additionally, Tyr improves sex hormone balance, oxidative stress levels, and inflammatory status, ultimately Oenhancing sperm concentration and restoring testicular tissue architecture in oligozoospermia. Future studies will focus on cellular‑level investigations to elucidate the specific effects of Tyr on different testicular cells.