PCR primers used for generation of BHV-1 U_L 49.5 mutants and for colony identification.

aBHV-1 UL 49.5-specific sequences are shown in uppercase letter; the italicized and italicized-underlined sequences are complementary to each other in reverse orientation. Nucleotides in lowercase indicate the pEPkan-S-specific sequences. bPrimers used for identification of BHV-1 UL 49.5 mutants from selected kanamycin-sensitive colonies by PCR. The bold letters indicate the complementary sequences, in reverse orientation, corresponding to the deleted UL 49.5 nucleotides.