Mouse Embryonic Fibroblasts (MEFs) Adipogenic Potential: A Comprehensive Transcriptome Analysis

Exploration into the dynamic process of adipogenesis in MEF cells via transcriptome analysis RNA isolates of MEF cells extracted at different timepoint and conditions. Timepoints included day 0,3, and 5. Conditions, for day 3 and 5, included adipocyte differentiating condition (incubation with basal growth media supplemented with 0.5 mM 3-isobutyl-1-methylxanthine , 10 μg/mL insulin, 1 μM dexamethasone, and 10 μM rosiglitazone) and non-differentiating condtion (basal growth media DMEM 10%FBS).