Human T-cell dysfunction induced in repeatedly stimulated cultures.

We report the changes in mRNA profile of quiescent, activated and dysfunctional human CD8+ T cells in culture according to a protocol of weekly polyclonal stimulations relevant to immunotherapy. Overall design: mRNA profile of quiescent enriched CD8+ T cells compared to cells after one stimulation with anti-CD3/CD38 beads or 5 to 7 rounds of weekly stimulations.