File S1 - Transgelin Up-Regulation in Obstructive Nephropathy

Methodology of 2D gel electrophoresis for obtaining comparisons of renal parenchyma alterations in the UUO model. Figure S1, Expression of transgelin 1 and transgelin 2 mRNAs in the hypertensive model. Real Time PCR was performed at the different time intervals indicated with the appropriate primers. The asterisk denotes statistical significance at a level of p<0.05. Figure S2, The specificity of nuclear staining of Transgelin. Figure 2A–C shows the staining of the negative control section of a ligated animal. The DAPI is shown in blue (2A), the staining of the secondary Cy2 anti-rabbit antibody is shown in figure 2B and the colocalization of them is presented in figure 2C. Figure 2D–F shows the staining of transgelin in a ligated animal. In 2D, the DAPI is shown in blue, in 2E the nuclear staining of transgelin is presented and in 2F, the colocalization of DAPI with transgelin.
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