The structure of gene networks that constrain and promote diversification in Heliconius melpomene

Comparison of whole genome and tiled gene expression between different colour pattern races of H. melpomene in developing wings. A total of 96 ds-cDNA samples labelled with Cy3 were hybridized onto 8 custom-designed Roche NimbleGen 12x135K format microarrays (Roche NimbleGen) for a total of 96 identical subarrays with 135,000 probes of 60 bp each and each hybridising a separate sample. The number of samples corresponded to two races, four stages and four tissue sections (forewing proximal, forewing distal, hindwing and eye), all of them with three independent replicates.