Transgenesis of mammalian PABP reveals new role of mRNA polyadenylation in general stress response in Escherichia coli

To determine the total mRNA polyadenylated in E.coli, we transexpressed mammalian nuclear poly(a) binding protein in E.coli through plasmid and chromosomal integration. RNA Seq analysis revealed general upregulation of around 600 genes commonly in between chrosomal MG PABP and plasmid PABP. 32% of the commonly upregulated genes fall into stress response mRNAs. E.coli K-12 MG 1655 cells were transformed with pBAD PABP for plasmid based PABP expression. Chromosomal integration of PABP was achieved using λ phage. Cells were grown up to OD600=0.5 and is harvested to isolate RNA for the same.