Expression analysis of microRNAs in INT-HA-induced M2-like macrophages and untreated macrophages (M0)

Using the highly sensitive miRNA microarray, we screened 107 up-regulated microRNAs and 33 down-regulated microRNAs in the INT-HA-induced M2-like macrophages and we explored the functions of these miRNAs in macrophage polarization. The enrichment results indicated that these miRNAs might participate in the process of macrophage polarization. Furthermore, the quantitative real-time polymerase chain reaction results showed that miR-935 may play an important role in M2 macrophage polarization which was activated by INT-HA. In this study, primary human macrophages derived from peripheral blood monocytes (n=3) were treated with 50 μg/ml INT-HA for 72 h. The miRNA expression profiling was acquired and the function of the abudant miRNAs in INT-HA-induced M2-like macrophages were analyzed by bioinformatic methods,finally,miR-935 might be involved in the regulation of macrophage polarization, a potential therapeutic target for antitumor treatment.