Global and region-specific rules of airway epithelial development

Lineage tracing experiment using a airway secretory specific Cre driver mouse. Airway secretory (club) cells were labelled from the beginning of the expression of the pansecretory marker Scgb1a1, at embryonic day 16.5 (E16.5) and samples were collected in four developmental stages (E19.5, P2, P21 and P60). Samples were collected right before activation of the Cre reporter, at E16.5, and served as control. We were interested to characterize the transcriptomic changes and uncover the molecular mechanisms that control the differentiation and maturation of the secretory cell lineage. Overall design: Airways epithelial secretory cells from Scgb1a1CREERT2;Rosa26R-fGFP mice were isolated by Fluorescence-activated cell sorting (FACS) according to the presence of GFP, at E19.5, P2, P21 and P60 stages. E16.5 stage cell samples were isolated by FACS based on Epcam and CD45 (EpCam positive and CD45 negative cells were selected) expression.