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Expression analysis of wt and maternal Trim28 mutant E3.5 embryos. Mus musculus

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NIAID Data Ecosystem2026-03-09 收录
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We performed RNAseq on 4 individual E3.5 control and maternal Trim28 blastocysts respectively in order to detect transcriptional differences amog the 2 cohorts which are to be linked to epigentic reprogramming defcts at early stages. Overall design: mRNA isloated from E3.5 embryos was reverse transcribed into cDNA using the SMART-Seq® Ultra® Low Input RNA Kit (Clontech), followed by sequencing library preparation using the Nextera DNA Library Preparation Kit (Illumina) according to manufacturers instructions.

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2016-09-30
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