Escherichia coli heat shock translational profiling. Escherichia coli str. K-12 substr. MG1655

Cellular protein levels are dictated by the balance between gene transcription, mRNA translation and protein degradation. Cells must manage their proteomes during stress: one way in which they may, in principle, do so is by differential translation. We used ribosome profiling to directly monitor translation in E. coli at 30°C and investigate how this changes after 10-20 minutes of heat shock at 42°C.