Urine sample storage after collection at ultra-low-temperature (e.g., −80 °C) is normally required for comparative metabolome analysis of many samples, and therefore, freeze–thaw cycles (FTCs) are una
Metabolomic analyses of urine samples from 50 Hunner-type IC/BPS patients and 30 healthy controls were performed at Shanghai Applied Protein Technology Co. Ltd. (Shanghai, China). 10 µL of supernatant
