Single-cell transcriptomic reveals heterogeneous feature of myeloid-derived suppressor cells in newborns

In order to gain deep insights into the molecular characteristics and heterogeneity of MDSCs in human newborns, low-density CD11b+HLA-DR-/low immature myeloid cells from the peripheral blood of term infants, preterm infants, and adults control were subjected to single-cell RNA sequencing (scRNA-seq). Peripheral blood samples were collected from full-term, preterm and adult controls. Single-cell suspensions of peripheral blood mononuclear cells (PBMCs) were obtained by centrifuging human blood samples on a Ficoll gradient. The strategy for MDSCs was CD11b+HLA-DR-/lo. Live immature myeloid cell in RPMI1640 supplemented with 20% FBS were sorted in a BD FACSAria III cell sorter (BD Biosciences).