<i>Chlamydomonas</i> strains used in this study and presence of discussed features.

Some of the tomograms from these strains were previously used with different image processing methods to analyze other axonemal complexes [30], [37], [51]. aStrain provided by Raqual Bower and Mary E. Porter (University of Minnesota). The N-DRC defective pf2 mutant strain [36] was rescued with a GFP-tagged WT PF2 gene. The resultant strain, pf2-4::PF2-GFP, is structurally and phenotypically indistinguishable from WT. bStrain provided by Douglas Tritschler and Mary E. Porter (University of Minnesota). The N-DRC defective ida6 mutant strain [78] was rescued with a GFP-tagged WT IDA6 gene and the resulting ida6::IDA6-GFP strain has the same motility and structure as WT. cStrain provided by Douglas Tritschler and Mary E. Porter (University of Minnesota). The N-DRC defective ida6 mutant strain [78] was rescued with a SNAP-tagged WT IDA6 gene. The resultant strain, ida6::IDA6-SNAP, is structurally and phenotypically indistinguishable from WT.