Table S1 - The Accuracy, Feasibility and Challenges of Sequencing Short Tandem Repeats Using Next-Generation Sequencing Platforms

Summary of microsatellite variant data and statistics from Sanger, GS-FLX and Illumina platforms. Indel variant number refers to the number of repeat units deleted or inserted; negative number refers to a deletion; positive number refers to an insertion. Var refers to the observed number of reads for the particular variant, while non-var refers to the number of remaining reads covering the variant position; i.e. total coverage minus variant reads at the given position. P used refers to a standard chi-square test, except when (i) expected read counts were less than one and we used Monte-Carlo simulation to calculate P values or (ii) the observed number of reads minus the expected number was ≥ the expected number and we used a log-likelihood ratio goodness of fit test. P values for the Illumina platform are for three-way comparisons between Sanger, Illumina and 454-sequencing. Significance: * statistically significant values unadjusted for multiple tests (P<0.05), ** statistically significant values after Benjamini-Hochberg correction for multiple tests, *** statistically significant values after Bonferroni correction.
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