RNA-seq in QGY-7703 cell line before and after PRMT7 knockdown

To explore the mechanism by which PRMT7 regulates HCC cell malignant phenotypes, we performed RNA-seq in QGY-7703 cells before and after PRMT7 knockdown to identify the affected signaling pathways. 296 differentially expressed genes were identified in PRMT7-downregulated cells (Figure 6A) and these genes were enriched in cancer-related pathways including MAPK signaling pathway, p53 signaling pathway, and FoxO signaling pathway (Figure 6B), as revealed by DAVID KEGG analysis. Overall design: RNA-seq was constructed in QGY-7703 cells without PRMT7 knockdown and cells with PRMT7 knockdown by two short hairpin RNA (shRNA), shRNA1 and shRNA2, respectively.