Real time quantitative PCR analysis of serum samples obtained from transgenic and wildtype mice. Real time quantitative PCR analysis of serum samples obtained from transgenic and wildtype mice

Wildtype mice and Autotaxin-overexpressing transgenic mice, both with and without spontaneous tumor formation, were maintained in a colony. Serum samples from mice in the aforementioned three groups were collected and a random subset of mice were selected for microarray analysis. RNA was extracted from serum and subsequently cDNA was generated and run on two 384-well plates probing for 373 viable targets, including microRNAs, control probes and interplate calibrators. Raw Ct values obtained from qPCR analysis were analyzed using GenEx and graphed using GraphPad Prism. Overall design: microRNA expression analysis was performed with RNA extracted from serum samples of wildtype and Autotaxin transgenic mice with and without spontaneous tumor formation.