Characterization of peripheral blood TCR in patients with Type 1 Diabetes Mellitus by BD Rhapsody™ VDJ CDR3 Assay

We used the latest technology, BD Rhapsody, to analyze the pairing of a and ß chains that constitute the TCR of PBMCs from patients with type 1 diabetes at the single-cell level. Overall design: We used single-cell VDJ sequencing using the latest technology, BD Rhapsody, to identify the TCR sequences of autoreactive T cells characteristic of Japanese type 1 diabetes mellitus (T1DM) and to clarify the pairing of TCR of peripheral blood mononuclear cells from four patients with T1DM at the single-cell level.