RNA-seq of mouse embryonic fibroblasts(MEFs) with knock-out of SERF2 compared to control MEFs

Serf2 has been identified as a potential modifier of aggregation in the case of several disease-related aggregation-prone proteins, such as amyloid-beta, alpha-synuclein and huntingtin. However, it's endogenous biological function remains unknown. Therefore, we generated Serf2-/- mice using the cre-lox system to explore the endogenous role of Serf2. As the knockout of Serf2 appeared to cause perinatal lethality, we isolated embryonic fibroblasts at embryonic day 13.5 from Serf2+/+ and Serf2-/- embryo's and generated monoclonal cell lines. We compared these cell lines through RNA-sequencing to investigate the role of Serf2 in embryonic development.