RNA-sequencing of mouse melanoma cell lines WT31, B16F10 luc2, RET, D4M and HCmel12

In vitro cultured, sub-confluent (50 %) mouse melanoma cell lines WT31, B16F10 luc2, RET, D4M and HCmel12 were analyzed by RNA-seq. All used melanoma cell lines were from murine origin. The melanoma cell line B16F10 luc2 was purchased from Perkin Elmer (MA, USA). RET1 melanoma cells were generated from metallothionein-I (MT)/RET transgenic mice and kindly provided by V. Umansky (German Cancer Research Center (DKFZ), Heidelberg, Germany). The transformed melanoma cell line WT31 was derived from Tyr∷NrasQ61K/°; INK4a−/− mice and was a generous gift from O. Sansom (Beatson Institute for Cancer Research, Scotland). HCmel12 melanoma cells were established from a primary melanoma in HGF-CDK4(R24C) mice and kindly provided by T. Tüting (University of Magdeburg, Germany). The Dartmouth murine mutant malignant melanoma (D4M) is derived from Tyr::CreER;BrafCA;Ptenlox/lox mice and was generously provided by C. E. Brinckerhoff (Geisel School of Medicine at Dartmouth, NH, USA). After thawing the cells were not passaged more than three times. Thereafter, RNA was extracted at sub-confluency (50%) and prepared for RNA-sequencing. The library preparation and the sequencing with an Illumina HiSeq 4000 sequencing system (Illumina, CA, USA) were then performed by BGI (Hongkong, China).