Triplication of the Ifnr locus is necessary for increased incidence of heart malformations in a mouse model of Down syndrome.

Images of haematoxylin and esoin stained sections of embryos from the Dp16 mouse model of Down syndrome, Dp16 corrected for Ifnr dosage (Dp162xIfnrs, Norm), and wildtype controls (WT), at E15.5.  Upon harvest, embryos were fixed at 4°C overnight in 2% paraformaldehyde diluted in 1x PBS stored in 70% ethanol before embedding in paraffin. Embedded embryos were sectioned transversely at 7 µm thickness using a LEICA RM 2155 Rotary Microtome. Serial sections were collected then haematoxylin and eosin Y (H&E) staining performed, followed by imaging with a Keyence BZ-X710 All-in-One Fluorescence Microscope. The investigators who sectioned embryos and performed H&E analysis were blind to embryo genotype.