Targeting BMI-1 to deplete antibody-secreting cells in autoimmunity

We measured genes expression differences in antibody secreting cells from LynKO mice in following treatment with a BMI-1 inhibitor (PTC-028) or a vehicle control. BMI-1 inhibition lead to a reduction in antibody secreting cells in LynKO mice and in humans donors. We performed RNA sequencing to understand the impact of BMI-1 inhibition on antibody secreting cells in LynKO mice. Antibody secreting cells (B220loCD138hi) were sort purified from LynKO mice 4 days after 12 consecutive days of PTC-028 treatment or vehicle control. RNAseq was undertaken on these cells.