Effects of imatinib on human islet gene expression as assessed by microarray analysis.

Human islets in groups of 1000 were exposed to DMSO (control) or to 10 µM of Imatinib for 4 hours. Following treatments, RNA was isolated, converted into cRNA and hybridized to Oligo GEArray Human Signal Transduction Pathway Finder Microarray filter according to the instructions of the supplier. The filters were scanned in a Kodak 4000MM Image station. Results from two independent experiments were analyzed by measuring the optical densities of the spots using the Kodak Molecular Imaging Software. Optical densities were normalized to housekeeping genes included on the filter (ribosomal protein S27a, GAPDH, beta-actin). Table shows the mean fold induction from the two separate experiments and only genes increased with more than 30% are shown.