Transcriptome map of plant mitochondria reveals islands of unexpected transcribed regions (CBX156). unidentified

The mitochondrial genome size of Nipponbare, the rice cultivar commonly used in molecular biology, is estimated at 490 520 bp [02]. Tiling probes of 60 mer were designed for 374,866-bp non-redundant sequences after discarding large duplicated regions of >10 000 bp. Total of 373,924 probes were designed to cover the 374,866 non-redundant region. Mitochondrial RNA was prepared from calli or etiolated-seedlings and hybridized against the tiling probes after biotin labeling upon reverse transcription by random primers.