circRNA-Panel seq data of phloem and leaf circRNAs from Brassica napus
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This dataset belongs to the publication "Characterizing the circular RNA landscape in phloem sap of Brassica napus". We performed Nanopore circRNA-Panel sequencing targeting 14 different circRNAs from two biological replicates of phloem sap and leaves from B. napus. Here, we provide bigwig files (CPM-normalized) generated from Minimap2 alignments of each of the sequenced barcodes to the B. napus genome assembly AST_PRJEB5043_v1 release-59. Each barcode resembles one circRNA target from one biological replicate from either leaves or phloem. CircRNAs amplified from phloem samples have barcodes between 1-20 and 41-60, circRNAs amplified from leaf samples have barcodes from 21-40 and 61-80. Additionally, we provide the bed-files generated by CIRI-long (by running circRNA identification, Isoform collaps and output visualization), combining the biological replicates. These bed-files provide an overview of identified circRNA isoforms from phloem and leaf samples. Fastq-files to these processed files can be found in the Sequence Read Archive (SRA) under the accession number PRNJA1267588.



