Additional file 1 of Interaction of porcine circovirus-like virus P1 capsid protein with host proteins

Additional file 1: Figure S2. Detection of self-activation of the bait protein. TheAH109 yeast cells that were cotransformed with pGADT7 and pGBKT7-ORF1, pGADT7-LargeT and pGBKT7-p53 (as a positive control), pGADT7-LargeT and pGBKT7-laminC (as a negative control), were plated on SD-TL (2a) and SD-TLHA medium (2b) fortheauto activation test.Yeast co-transfected with plasmids pGADT7 and pGBKT7-ORF1 cannot grow on SD-TLHA medium and did not turn blue intheβ-galactosidase assay, indicating that pGBKT7-ORF1 does not autonomously activatethereporter genes in yeast cells without a preyprotein (2c).