RNA-seq of the mouse myoblasts cell line C2C12 cultured in four temperature-treatment conditions

This experiment aims at investigating the effects of heat-stress on transcriptomic changes of the muscle cells in in-vitro model using the mouse myoblast cell line C2C12. Four incubation temperatures were set as a treatment condition at 35°C (cold stress), 37°C (control), 39°C (mild heat stress) and 41°C (severe heat stress). The cells were cultured under the treatment conditions for 72 hours prior to harvesting the cells for RNA extraction. The experiment was done in three independent replications.