Gene expression data comparing HIV exposed and HIV unexposed freshly isolated umbilical cord blood derived CD34+ haematopoietic stem/progenitor cells

Umbilical cord blood (UCB) is a donor source for haematopoietic stem cell transplantation but only HIV unexposed and uninfected cord blood has been used to date. HIV exposed and uninfected cord blood could potentially be considered as a donor source in HIV endemic countries, provided the functioning of these CD34+ Haematopoietic stem cells (HSPCs) is comparable to HIV unexposed cells. We performed gene expression analysis on umbilical cord blood CD34+ HSPCs to determine if any differences exist between HIV unexposed and HIV exposed cohorts with mothers taking newer antiretroviral drugs. Umbilical cord blood was obtained from term HIV exposed and unexposed infants. After obtaining a pure mononuclear cell population, magnetic isolation was employed to enrich for CD34+ HSPCs. RNA was extracted from the CD34+ HSPCs on the day of isolation. This was followed by hybridisation on Affymetrix microarrays.