Regional perfusion, differences in amino-acid fluxes and citrulline concentration in ND14 Ass-Con and Ass-KO/I mice.

Panel A: Blood flow in portal vein (“Portal Drained Viscera”), hepatic artery (liver), hepatic vein (viscera), renal arteries (kidneys), femoral arteries (hindquarter) and brain as assessed by microsphere distribution after intracardiac injection. Panel B: Significant differences in the flux of amino acids across the splanchnic region, portal drained viscera, liver, and kidneys of ND14 Ass-KO/I compared to ND14 Ass-Con mice. C: Significant differences in the flux of amino acids across the splanchnic region, portal drained viscera and liver of ND14 Ass-KO/I compared to ND35 Ass-Con mice. In panels B and C, dark gray fields represent differences with P ≤ 0.05. Differences with 0.05 ≤ P ≤ 0.10 (light gray) are also shown, because the power of our calculations was weakened by the fact that we could take only one blood sample from a vessel in each ND14 pup. Panel D: Average concentration of citrulline in the respective vessels (cf. also Table S3).