Additional file 2: of An IMD-like pathway mediates both endosymbiont control and host immunity in the cereal weevil Sitophilus spp.

imd and relish inhibition by RNAi in S. zeamais (a) and S. oryzae (b). imd and relish expressions were measured by RT-qPCR in bacteriomes and carcasses. Tissues were dissected 6 h following either PBS or TCT injection, 6 days after gfp, imd, or relish dsRNA injection. Gene expression was normalized by the geometric mean of two housekeeping gene expressions, rpl29 and mdh. Raw data (mean ± SD) as well as inhibition percentages are provided. Percentages were calculated as follows: % = 1 − imd RNAi or relish RNAi gfp RNAi × 100 $$ \%=\left(1-\frac{imd\ \mathrm{RNAi}\ \mathrm{or}\ relish\ \mathrm{RNAi}\ }{gfp\ \mathrm{RNAi}\ }\right)\times 100 $$ ; using the mean of five independent measurements for each value. Asterisks indicate a significant difference between a condition and its corresponding gfp RNAi control based on a Welch’s t test (*p < 0.05; **p < 0.01; ***p < 0.001). (XLSX 11 kb)