MicroRNA miR-145-5p regulates cell proliferation and cell migration in colon cancer by inhibiting chemokine (C-X-C motif) ligand 1 and integrin α2

Colon cancer (CC), which has high morbidity and mortality, can be regulated by microRNAs. This study aimed to investigate the regulatory function of microRNA miR-145-5p in CC cells. Bioinformatics analysis was used to screen key genes in CC. The expression of miR-145-5p, chemokine (C-X-C motif) ligand 1 (CXCL1), and integrin α2 (ITGA2) in CC was confirmed by quantitative reverse transcription polymerase chain reaction and western blotting. After cell transfection, changes in proliferation and migration in CC cells were detected using the cell counting kit-8 (CCK-8), colony formation assay, and wound healing assay. A luciferase assay was conducted to confirm the interactome of miR-145-5p, CXCL1, and ITGA2 in CC cells. Bioinformatics analysis confirmed that CXCL1 and ITGA2 were key genes in CC. After performing several cell functional experiments, the results confirmed that upregulation of miR-145-5p attenuated proliferation and migration of CC cells. Luciferase assay and western blotting confirmed that CXCL1 and ITGA2 were targets of miR-145-5p, and their expression in CC could be suppressed by miR-145-5p. In conclusion, miR-145-5p is a tumor suppressor in CC and can inhibit the expression of CXCL1 and ITGA2.

结肠癌（CC），作为一种发病率与死亡率均较高的恶性肿瘤，其发生发展可受微RNA的调控。本研究旨在探究微RNA miR-145-5p 在结肠癌细胞中的调控功能。通过生物信息学分析，筛选出结肠癌中的关键基因。采用定量逆转录聚合酶链反应和蛋白质印迹法对 miR-145-5p、趋化因子（C-X-C 结构域）配体 1（CXCL1）和整合素 α2（ITGA2）在结肠癌中的表达进行验证。细胞转染后，利用细胞计数试剂盒-8（CCK-8）、集落形成实验和伤口愈合实验检测结肠癌细胞的增殖和迁移变化。通过萤火虫荧光素酶实验验证了 miR-145-5p、CXCL1 和 ITGA2 在结肠癌细胞中的互作网络。生物信息学分析证实 CXCL1 和 ITGA2 是结肠癌中的关键基因。通过一系列细胞功能实验，证实 miR-145-5p 的上调可抑制结肠癌细胞的增殖和迁移。萤火虫荧光素酶实验和蛋白质印迹法进一步证实 CXCL1 和 ITGA2 是 miR-145-5p 的靶基因，且 miR-145-5p 可通过抑制其在结肠癌中的表达来实现抑制。综上所述，miR-145-5p 在结肠癌中发挥抑癌基因的作用，并可抑制 CXCL1 和 ITGA2 的表达。