RNF111 ubiquitinates SUMOylated XPC

SUMOylated XPC is recognized by the SUMO-targeted ubiquitin ligase RNF111 (Arcadia) that, together with the E2 ubiquitin conjugating complex of UBE2N (UBC13) and UBE2V2 (MMS2), generates K63-linked polyubiquitin chains on XPC (Poulsen et al. 2013) to efficiently release XPC from UV lesions (van Cuijk et al. 2015). The release of K63-polyubiquitinated XPC occurs from GG-NER pre-incision complexes that contain TFIIH and XPA and promotes optimal access/binding of ERCC5 (XPG) endonuclease to the pre-incision complex (van Cuijk et al. 2015). Successful binding of ERCC5 endonuclease 3' to the damage facilitates binding of the ERCC1:ERCC4 (ERCC1:XPF) endonuclease and progression of the NER reaction.

SUMO化型XPC被SUMO靶向泛素连接酶RNF111（Arcadia）识别，该酶与UBE2N（UBC13）和UBE2V2（MMS2）组成的E2泛素连接酶复合物共同作用于XPC，生成K63连接的多泛素链（Poulsen等，2013），以高效地从紫外线损伤中释放XPC（van Cuijk等，2015）。K63多泛素化XPC的释放发生在含有TFIIH和XPA的GG-NER前切割复合物中，并促进ERCC5（XPG）内切酶对前切割复合物的最佳接近/结合（van Cuijk等，2015）。ERCC5内切酶3'端对损伤的成功结合，有利于ERCC1:ERCC4（ERCC1:XPF）内切酶的结合，并推动NER反应的进行。