青脆枝癒合組織誘導、生長與喜樹鹼分析

以青脆枝之胚、子葉、下胚軸及葉片為試驗材料，利用不同植物生長素之培養基誘導其癒合組織的發生及生長。結果發現，以MS基本培養基添加NAA或TDZ對癒合組織誘導及生長效果較好，而培植體則以胚、子葉、下胚軸優於葉片；NAA之癒合組織容易引起不定根再生，而TDZ則較偏向不定芽或胚性組織之再生。取不同植物生長素生產之子葉癒合組織分析其喜樹鹼含量，以NAA誘導之癒合組織含有較高喜樹鹼，可達330.65 ppm，結果顯示青脆枝癒合組織喜樹鹼含量取決於不定根的再生。

Embryos, cotyledons, hypocotyls and leaf blades of Nothapodytes foetida (Qingcuizhi) were used as experimental materials to induce callus formation and promote its growth using media supplemented with various plant growth regulators (PGRs). The results showed that MS basal medium supplemented with NAA or TDZ yielded better effects on callus induction and growth, and the explants including embryos, cotyledons and hypocotyls outperformed leaf blades. Calli induced by NAA tended to readily regenerate adventitious roots, whereas TDZ-supplemented media more frequently induced the regeneration of adventitious buds or embryogenic tissues. We analyzed the camptothecin content in cotyledon-derived calli induced by different PGRs, and found that calli induced by NAA contained a higher camptothecin concentration, up to 330.65 ppm. The results indicated that the camptothecin content in calli of Nothapodytes foetida (Qingcuizhi) depends on adventitious root regeneration.