Chlorophyll a and phytoplankton assemblage data from temperature and nutrient interaction incubation experiments on deck during RRS Discovery cruise DY110 (AMT29).

This dataset contains the Chlorophyll a concentration, picophytoplankton abundance, and abundance, cell area, and cell volume of large nano- and micro-phytoplankton from temperature and nutrient incubation experiments. The samples used for the experiments were taken along the Atlantic Meridional Transect (AMT) during RRS Discovery cruise DY110 (AMT29) between the 13th October 2019 and 25th November 2019. Samples were taken from the incubation at the start and end of the incubation period (0 and 96 hours) for analysis of chlorophyll a concentration and abundance and cell volume of large nano- and micro-phytoplankton. Chlorophyll a concentration was determined with a Turner Designs Trilogy fluorometer. Large nano- and microphytoplankton were analysed by automated digital imaging using a Yokogawa Fluid Imaging Technologies FlowCAM 8400. Samples were taken from the incubation at 24-hour intervals to determine the abundance of picoeukaryotes and cyanobacteria Prochlorococcus and Synechococcus by flow cytometry using a Becton Dickinson FACSort equipped with an air-cooled laser providing blue light at 488 nm. These experiments aimed to characterise the interactive effect of simultaneous changes on temperature and nutrient availability in tropical and subtropical phytoplankton assemblages. These data were collected by scientists from the University of Vigo, for the project: TRIATLAS South and Tropical Atlantic climate-based marine ecosystem prediction for sustainable management (H2020). These data are available under the most recent version of the NERC Open Government Licence.