Spatial and single-cell transcriptomics uncover brassinosteroid-mediated coordination of sepal elongation in Arabidopsis

code_drmy1_Brassinosteroid_single_cell_spatial_project Scripts for data analysis: aggregated_seurat_analysis_CCremoved_v5.R Finding_hormone_genes.R GO_Analysis_script_aggregated_pseudobulk.R Graphing_sepal_ratios_BL-single-timepoints.R Graphing_sepal_ratios-BR_Mutants.R Graphing_sepal_ratios-BRZ-single-timepoint.R GSEA_hormone_genes.R Pseudobulking.R remake_dotplots.R Restarting_analyses_HORMONEDOTPLOTS.R Seurat_Deconvolution_v2.r visium_graphs.R Fig_5_BES1_ratiometric_WT_vs_drmy1 WT and drmy1 expressing pUBQ10::H2B-mcherry (magenta)/pUBQ10::BES1-ypet (green) Fig_5_BES1_ratiometric_reporter_Cycloheximide_treatment Day 6 mock and 2 μM cycloheximide treated buds expressing the BES1/H2B ratiometric reporter. Fig_6_double_mutant_analysis Confocal images of single brassinosteroid mutants bzr1-1D and det2, as well as double mutants with drmy1 expressing the membrane marker 35S::RCI2A-mCitrine. Heterozygous bzr1-1D lines are shown as representative images to demonstrate that the gain-of-function phenotype is visible with just a single copy of bzr1-1D. Homozygotes had visually similar phenotypes. Fig_6_Brasinolide_single_time_point Confocal images of WT and drmy1 flower buds treated for 6 days with either 400 nM brassinolide (BL), or mock treatments. Buds are either expressing the membrane marker 35S::RCI2A-mCitrine or are stained with 0.1 mg/mL propidium iodide. Fig_6_Brasinozole_single_time_point Confocal images of WT and drmy1 flower buds treated for 6 days with either 50 uM brassinazole (BRZ), a brassinosteroid biosynthesis inhibitor, or mock treatments. Buds are either expressing the membrane marker 35S::RCI2A-mCitrine or are stained with 0.1 mg/mL propidium iodide. Fig_7_live_imaging_drmy1_WT_BL_BZR Live imaging of developing flower buds every 12 hours of wild type or drmy1 either mock treated, treated with 400 nM brassinolide (BL), or treated with 50 μM brassinazole (BRZ).