Czech Butterfly Barcoding

Dataset for Czech Butterfly BarcodingThis dataset consists of separate nexus files for each butterfly species covered in the Czech Republic Butterfly barcoding scheme.Nexus files include newly generated sequences from the Czech Republic, and database data from BOLD (Ratnasingham &amp; Hebert 2007) (downloaded on 12 October 2022), which were georeferenced and sequenced from the 5' end. We manually aligned the samples. We prepared the nexus files which serve as inputs for both POPART programme (Leigh &amp; Bryant 2015; available from https://popart.maths.otago.ac.nz/) and rhierbaps R package (Tonkin-Hill et al. 2018). Both results of POPART and rhierbaps were checked visually and the dataset was cleaned to omit database samples which were either shorter than 500 bp, or potentially misidentified (dissimilarity of sequences represented by long branches in haplotype networks was evaluated using the BLAST algorithm, Altschul et al. 1990, in the NCBI database). After such cleaning, the final dataset (samplelist_metadata.xlxs) and final nexus files, presented here, were produced.<br>Altschul, S.F., Gish, W., Miller, W., Myers, E.W. &amp; Lipman, D.J. (1990). Basic local alignment search tool. J. Mol. Biol., 215, 403–410.Leigh, J.W. &amp; Bryant, D. (2015). popart: full-feature software for haplotype network construction. Methods Ecol. Evol., 6, 1110–1116.Ratnasingham, S. &amp; Hebert, P.D.N. (2007). bold: The Barcode of Life Data System (http://www.barcodinglife.org). Mol Ecol. Notes, 7, 355–364.Tonkin-Hill, G., Lees, J.A., Bentley, S.D., Frost, S.D.W. &amp; Corander, J. (2018). RhierBAPS: An R implementation of the population clustering algorithm hierBAPS. Wellcome Open Res., 3, 93.