小鼠杏仁中央核表达mu型阿片受体的神经元单细胞全脑投射谱

本数据集展示了小鼠全脑范围内中央杏仁核（CeA）中表达mu型阿片受体（MOR）的神经元（CEAMOR神经元）单细胞投射图谱。CeA神经元是杏仁核的主要输出神经元，具有多个下游靶脑区。我们通过单细胞投射组分析，基于投射模式对这些神经元进行全面分类，重建了67个CEAMOR神经元的全脑轴突投射，并将其配准至Allen小鼠脑公共坐标框架（CCFv3）。基于轴突投射模式对CEAMOR神经元进行分类，鉴定出4种不同亚型。分析结果显示第一类亚型具有向后脑多个亚区投射的特点；而第二类亚型主要投射到以终纹床核为代表的前脑区域；第三和第四类亚型则主要表现局部投射模式。单细胞投射谱分析显示，同一亚型的CEAMOR神经元投射到多个脑区，并具有独特的神经元特异性投射偏好。提示，不同投射亚型的CEAMOR神经元以及同一神经元的不同轴突分支可能执行不同功能。

Single-cell projection atlas of mu-opioid receptor (MOR)-expressing neurons (CEAMOR neurons) in the central amygdala (CeA) across the entire mouse brain is presented in this dataset. CeA neurons are the major output neurons of the amygdala, projecting to multiple downstream brain regions. Using single-cell projectionomics analysis, we comprehensively classified these neurons based on their projection patterns, reconstructed whole-brain axonal projections of 67 CEAMOR neurons, and registered them to the Allen Mouse Brain Common Coordinate Framework (CCFv3). Classification of CEAMOR neurons based on their axonal projection patterns identified four distinct subtypes. Analysis results showed that the first subtype projects to multiple subregions of the hindbrain; the second subtype primarily projects to forebrain regions represented by the bed nucleus of the stria terminalis (BNST); the third and fourth subtypes mainly exhibit local projection patterns. Single-cell projection profile analysis revealed that CEAMOR neurons of the same subtype project to multiple brain regions and possess unique neuron-specific projection preferences. This suggests that CEAMOR neurons with different projection subtypes and distinct axonal branches of the same neuron may perform distinct functions.

搜集汇总

背景与挑战

背景概述

该数据集通过单细胞分辨率重建了小鼠杏仁中央核表达μ型阿片受体的67个神经元在全脑的轴突投射，并基于投射模式识别出四种亚型，分别具有不同的目标脑区偏好。这为理解CEAMOR神经元在神经环路中的结构和功能多样性提供了详细的解剖学基础。