PL measurements alpha syn KD 2022

Based on the research studies showing an altered PUFAs plasma membrane composition upon alterations to alpha synuclein expression levels, we hypothesised that reducing alpha syn in LUHMES dopaminergic neurons may have altered the plasma membrane composition. Phospholipid molecular species were therefore measured in LUHMES cells NT, Ctrl KD, asyn KD and ACSL4 KD under basal control or 20uM Arachidonic acid (AA) conditions. Here we report a selective and prominent reduction of ether-linked phospholipids (PC and PE) upon alpha syn and ACSL4 depletion. Such reduction in ether-PL may explain the ferroptosis resistance phenotype observed.