Characterization of human hippocampal neurons and interneurons using single-cell RNA-seq

The goal was to define the cellular heterogeneity in the human dentate gyrus (DG) and delineate this from the cellular architecture in the Cornu Ammonis (CA) region. Therefore, after isolation of NeunN-expressing nuclei from fresh-frozen hippocampal tissues (DG and CA), nuclei were used to generate single-cell transcriptomes using a commercial microwell-based assay. After library preparation and sequencing, the generated data were further processed and aligned against the reference genome. Gene expression matrixes are provided which can be further analyzed and overlaid with synaptic maps and organization, microanatomical and physiological data, and integrated for the generation of a human brain atlas.